RBS

Part:BBa_M36120:Design

Designed by: Alaina Shumate   Group: Stanford BIOE44 - S11   (2013-10-25)


5' Bicistronic UTR (strong) contains ATG start Codon.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to consider the strength in the design of this sequence. Because our protein ( human myoglobin) is not shown to be toxic to e. coli, we want in to be expressed in high numbers and thus we chose a strong BCD.


Source

This is based on research done in the paper "Precise and reliable gene expression via standard transcription and translation initiation elements."

References